Biological Evidence that Scrapie Agent Has an Independent Genome

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Biological evidence that scrapie agent has an independent genome.

There are many distinct strains of scrapie agent, identified by their relative incubation periods and quantitative and qualitative neuropathological properties in inbred mice of particular genotypes. When serially passaged under specified conditions of mouse strain, route of infection and dose of infectivity these properties are stable. However, they may change in a predictable manner if the pa...

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Although scrapie has been studied for many years, the nature of the scrapie agent remains obscure (14). In spite of a number of unusual properties, this agent has been generally considered to be a virus (9, 13). In a previous study, repeated daily injections of 6,000 units of interferon failed to protect mice challenged intracerebrally (ic) with about 5,000 median lethal dose (LD50) of the scra...

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Constructing a genome assembly that has the maximum likelihood

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The search for scrapie agent nucleic acid.

Despite decades of research, the identity of the scrapie agent has remained elusive. Recent studies have discovered much about the influence of the host genome upon scrapie infection, yet relatively little is known about the causative agent itself. The predominant hypothesis in the scrapie field (the prion hypothesis) argues that the disease is the result of an infectious protein and that nucle...

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The nature of the scrapie agent.

There now seems little doubt that the infective agent of scrapie cannot be accommodated within current concepts of virology/molecular biology. It is proposed: that the basic infective entity is a nucleic acid fragment (oligonucleotide) of some 40 bp coupled with specific (but host encoded) protein totalling approximately 10(5) daltons, a significant proportion of which is in the form of proteol...

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ژورنال

عنوان ژورنال: Journal of General Virology

سال: 1987

ISSN: 0022-1317,1465-2099

DOI: 10.1099/0022-1317-68-1-79